GeneTel Laboratories LLC

www.genetel-lab.com; info@genetel-lab.com; 1-877-248-4316

Custom Chicken and Rabbit Antibody Production Services

GeneTel provides low cost, high quality peptide and chicken antibody production services.  Followings are the prices and schedules.

Mouse monoclonal antibody production: Customer provide antigen (MAB0001) $5000, about 6 months

Mouse monoclonal antibody production: Peptide antigen made in-house (MAB0001P) $5200, about 7 months

Please email to inquire more info (info@genetel-lab.com)

MORE DETAIL FOR CUSTOM CHICKEN ANTIBODY PRODUCTION (Item# CA-0001)

Customer provides ready-to-immunize antigen

Chicken set up per hen                                                                                                              $15

Immunization*: Intramuscular (3 times)                                                                                       $10 each

Subcutaneous                                                                                                                            $10

Termination                                                                                                                               $15   

IgY isolation from 10 eggs (provide >500mg crude IgY)**                                                           $75

Daily maintenance fee (60-70 day)***                                                                                        $1 per day

Estimated total cost per hen is about $200, per project with 2 hens: $400.  Customer provide ready-to-immunize antigen

(Freight-Federal Express Priority overnight, customer pays for the shipment)

* All injections included Freund’s adjuvant, other adjuvants are available at an additional cost; four injections are normally recommended and at least 2 hens are recommended.

** One pre-immune egg and about 20 immune eggs are normally collected from each hen.  The immune eggs will be collected 1 weeks after the second immunization.

***A total of about 60 days is normally recommended.  Extended period can be requested with additional cost of $1 per day.  Daily maintenance includes care, feeding and veterinary care if necessary.

NOTE:  We will not accept radioactive, toxic, or infectious antigens.

Please send inquiry for custom antibody production in other species to: info@genetel-lab.com

ANTIBODY PURIFICATION

1.         Antibody Purification (AP-1)- Total IgY (about 50mg/egg): IgY will be purified to about 90% purity and protein concentration will be determined. $20/egg for individual egg, $10/egg for pooled eggs. Total cost is about $360 (12 x $20 = $240 for individual eggs, 12 x $10 = $120 for pooled eggs) for 500-1000mg IgY. 

2.   Antibody Purification (AP-2): This service includes antibody purification using Protein-A affinity chromatography. Price: $ 65 for 5-10 mg IgG (human IgG, mouse IgG, rabbit IgG and bovine IgG), $85 for 10-30 mg, $105 for 30-50 mg, $125 for 50-100 mg, $650 per g and $500 per g for > 2 g, For goat IgG, add 15% more.

3.   Antibody Purification (AP-3): This service includes antibody purification using Protein-G affinity chromatography. Price: $ 85 for 10-20 mg IgG, $105 for 20-40 mg, $135 for 40-60 mg, $175 for 60-100 mg, $925 per g, $750 per g for >2 g.

4.   Antibody Purification (AP-4): This service includes antibody purification using Protein-L affinity chromatography. Price: $ 90 for 10-20 mg IgG, $105 for 20-40 mg, $135 for 40-60 mg, $165 for 60-100 mg, and $750 per g.

5.   Antibody Purification (AP-5): This service includes antibody purification using Protein-LA affinity chromatography. Price: $ 95 for 10-15 mg IgG, $115 for 20-40 mg, $135 for 40-60 mg, and $180 for 60-100 mg, and $950 per g.

6.  Affinity Purification (AP-6):  This service includes antibody purification using protein, peptide or other molecules made affinity chromatography.  Price:  $600 for yield from 2-10mg.   We need about 5-10mg free peptide, ideally peptide with a cystein at C or N terminal, or 5-10mg protein (or fusion).

SDS-PAGE of Antigens

Antigen Purification - about 3 mg antigen needed for four injections of 2-3 hens. Customer provides information on protein concentration and buffer conditions. The purity of target protein must be >30%. Cost: $160 per antigen.

CONJUGATION TO CUSTOMER SUPPLIED ANTIGEN

Conjugation of up to 10 mg of antigen to two conjugates (KLH or BSA) - Cost: $150

Conjugation of same antigen to an additional carrier – Cost: $100

ANTIBODY TITRATION BY ELISA

TITRATION ASSAY IN 96 WELL MICROPLATE 50 - 100ug antigen needed per plate for coating and protein concentration must be provided. Need to know buffer conditions

$200 for first microplate, $150 for each additional microplate

WESTERN BLOT ASSAY

This is a four-lane-assays. Need 100ug antigen, approximate molecular weight of protein of interest, protein concentration and buffer conditions. Cost: $200 for first four lanes, $100 for each additional four lanes.

BIOTINYLATION

We provide low background secondary Abs such as bovine anti-IgY biotin and anti-IgY HRP (see Conjugates Section). These Abs are suitable for ELISA, Western Blot and IHC/ICC.  However, we also provide biotinylation service.  Cost: $150/5mg. 

CUSTOM PEPTIDE SYNTHESIS

We help customers to find and design the best antigenic peptides as immunogens. In general, 10-20 aa peptides are selected as antigens. Cost: $30 per aa for 10-20mg MAP (multiple antigenic peptide with lysine branches) peptides.

WESTERN BLOT ANALYSIS PROTOCOL

Western Blot Analysis Protocol

1.                  After transferring proteins from SDS-PAGE to nitrocellulose membrane, block the membrane with blocking buffer (5% nonfat milk in TBS) for 1 hour at RT, or overnight in 4^oC.

2.                  Dilute Abs accordingly in Abs dilution buffer (3% nonfat milk in TBST (TBS plus 0.1% Tween 20)) and add the Abs in each blot.

3.                  Shake at RT for at least 2 hour or keep in 4^oC overnight.

4.                  Empty the solution and wash at least 3X10 minutes with TBST with shaking.

5.                  Dilute conjugate in 3% nonfat milk/TBST (Genetel Anti-IgY AP 1:3000) and incubate blots with the conjugate for 1 hour, shaking.

6.                  Empty the solution and wash at least 4X10 minutes each with TBST.

7.                  Wash 1X 10 minutes with TBS.

8.                  Lay the membrane in a flat container and do color development using AP substrate. 

Buffer

TBS:

NaCl                                        8g                    KCl                                          0.2g

Trizma Base                             1.21g               D.I. Water                                1L

Adjust pH to 8.6 with 1M HCl

Blocking Buffer: TBS + 5% Nonfat Dry Milk.

TBST:

1L TBS

1 ml Tween-20 (Polyoxyethylene-Sorbitan Monolaurate)

Abs Dilution Buffer: TBST + 3% Nonfat Dry Milk

Note: Due to the nature of IgY sticky property, you may need to increase Tween-20 in your TBST.